In Vitro Functional Analysis
Creative Biolabs provides in vitro functional analysis for target validation of drug discovery process thus to deal with data reproducibility crisis which is a big obstacle for drug research and development and may lead to failure and high risk of investment. In vitro functional analysis can be used to reveal regulative characteristics of targets and the pathways in which they are involved. Our scientists can validate novel targets in different cell types such as stem cells, primary cells and cancer cells. Cell-based studies can provide in vitro models for early efficacy and safety assessment related to diseases.
Creative Biolabs offers a variety of in vitro functional analysis including but not limited to the following:
Cell Viability & Proliferation Assays
Cell viability assays are used to monitor the response and health of cells in culture after treatment with various stimuli which is necessarily evaluated prior to any cell-based study. Cell proliferation assays are used to monitor cell growth which plays an important role in regular tissue and cellular homeostasis for proper growth, development and maintenance of the organism by measuring the cytotoxicity, cell cycle and apoptosis.
Cell Migration & Invasion Assays
Cell migration is a central process in the development and maintenance of multicellular organisms. It contributes to many physiological processes, such as embryonic development, wound healing and immune response. Cell Invasion refers to the ability of cells to become motile and invasive cells can move through the extracellular matrix into neighboring tissues. Cell migration & invasion assays are important for understanding the pathological processes they are involved, such as tumor cell metastasis.
Transfection is the process of artificially delivering exogenous DNA or RNA into eukaryotic cells. It is a powerful tool used to study and control gene expression. The introduced nucleic acid can be transiently transfected into the cells and only expressed but not replicate for a limited period of time, or be stable transfected and integrated into the genome of the recipient. Transfection is a powerful analytical tool for studying the function and regulation of genes or gene products.
Three-Dimensional Cell Services
Three-dimensional (3D) cell culture is a technology that permits cells to grow or interact with their surroundings in all three dimensions by artificially creating an environment close to the living conditions in vivo. It can be used to study the behavior of cells in response to their environment. Compared to other cell culture methods, 3D cell culture characterized by novelty and convenient has been increasingly used, especially in preclinical pharmacokinetic and pharmacodynamic studies of drugs.
Phage display is one of the most powerful and widely used laboratory technique for the study of molecules interaction, such as protein-protein, protein-peptide and protein-DNA interactions. Theoretically, phage display is an exogenous gene expression method that connects genotype and phenotype. By inserting a gene encoding a protein of interest into a bacteriophage coat protein genes, interested protein will be displayed on the surface of the phage. Phage display can be used as a powerful tool for target validating in drug discovery, determination of interaction partners of a protein or in vitro protein evolution.
Creative Biolabs has a dedicated assay development team that will work closely with you to design customized assays. Our service will meet your specific needs fast at extremely competitive prices. If you need more information, please feel free to contact us at anytime. We look forward to working with you to help your drug research and development project succeed.